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1. Place 1.0gm of feces or 1ml of liquid stool in tube. Swab specimens may be inserted directly into the broth. 2. Emulsify the specimen thoroughly. 3. Incubate aerobically for six to eight hours at 35ºC. 4. Place one to two drops of the incubated broth onto selective plate media, such as MacConkey or Hektoen Enteric Agar and streak for ...

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